Antibody Validation
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Open iron metabolism antibody validation initiative

Our goal is simple — to provide a joint platform for researchers in iron metabolism to share their experience - both positive and negative - with some of the many commercially available antibodies.

Erythroferrone

Posted on May 13, 2021May 28, 2021 by Jan Krijt

Santa Cruz sc-246567 (anti-myonectin)

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Discontinued :(

TFR2

Posted on April 1, 2021April 19, 2021 by Jan Krijt

Abcam ab80194

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Functional antibody :)

phospho-SMAD 1,5

Posted on August 15, 2018April 25, 2021 by Jan Krijt

Abcam ab92698

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Functional antibody :)

HFE

Posted on May 22, 2018May 13, 2021 by Jan Krijt

Santa Cruz sc-18810

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Discontinued :(

Neogenin

Posted on May 29, 2017April 1, 2021 by Jan Krijt

R&D AF 1079

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Functional antibody :)

Transferrin Receptor 2

Posted on May 25, 2017April 1, 2021 by Jan Krijt

Alpha Diagnostics International TFR21-A

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Functional antibody :)

Hemojuvelin

Posted on May 3, 2017May 4, 2021 by Jan Krijt

R&D AF3634, AF3720

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Functional antibody :)

Ferroportin

Posted on May 3, 2017May 13, 2021 by Jan Krijt

Alpha Diagnostics International MTP11-A

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Functional antibody :)

Matriptase-2 (TMPRSS6)

Posted on May 3, 2017April 1, 2021 by Jan Krijt

Abcam 56182

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Functional antibody :)

Transferrin Receptor

Posted on September 25, 2023September 25, 2023 by Jan Krijt

Abcam ab214039

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Functional antibody :)

Ferroportin

Posted on September 21, 2023September 24, 2023 by Jan Krijt

Novus Biological NBP1-21502

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Not working :(

HFE

Posted on June 13, 2021June 13, 2021 by Jan Krijt

Invitrogen PA5-88983

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Not working :(

HFE

Posted on June 4, 2018May 20, 2021 by Jan Krijt

Santa Cruz sc-514405

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Not working :(

Matriptase-2 (TMPRSS6)

Posted on May 25, 2018April 1, 2021 by Jan Krijt

Sigma SAB2103628

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Not working :(

phospho-SMAD 1,5,9

Posted on May 23, 2018April 1, 2021 by Jan Krijt

Cell Signaling Technology  #13820

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Works only in vitro
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